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KMID : 1377020140110010075
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Tissue Engineering and Regenerative Medicine
2014 Volume.11 No. 1 p.75 ~ p.79
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Neonatal fibroblast affect the stemness of interfollicular epidermal cells by modulation of basement membrane
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Choi Hye-Ryung
Park Kyoung-Chan
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Abstract
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Fetal wounds reepithelialize rapidly but the mechanism is still unknown. In this study, the epidermal regenerative effects of neonatal fibroblast (n-fibroblasts) were compared to that of child fibroblasts (c-fibroblasts) using a skin equivalent model, which was constructed using interfollicular epidermal stem cells and transit amplifying cells, respectively. Results showed that n-fibroblasts increased the layers of epidermis when compared to c-fibroblasts. An increased number of p63 and PCNA positive cells were observed in SA/n-fibroblasts models {skin equivalent constructed with transit amplifying cells (SA cells) and n-fibroblasts} when compared to SA/c-fibroblasts models {skin equivalent constructed with SA cells and c-fibroblasts}. Expression of both β1 integrin and α6 integrin were increased by n-fibroblasts. The differentiation status was not significantly different. These findings suggested that n-fibroblasts can increase the stemness and proliferative potential of epidermal cells and n-fibroblast can be a source for cell therapy especially for chronic wounds.
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KEYWORD
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neonate, fibroblasts, epidermal stem cells, wounds
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